Mutation in Ompk35 are Evolution Pathway in Antibiotic Resistance

Abstract

OmpK35 is the major outer membrane porins of Klebsiella pneumoniae. This study was amid to determine the effect of mutation in Ompk35 in antibiotic resistance. This cross-sectional study was, performed on 250 clinical specimens collected from three major hospitals in wasit between. The presence of antibiotic resistance determinants was, investigated by polymerase chain reaction (PCR) method. The purified PCR products of ompk35 positive isolates were, sequenced to screen for mutations in porins. Among the types of mutations that were found in resistant isolates were the frameshift mutations, the mutations that occurred in the ompk35 gene, they are as follows:(78 G>R, 86          S>P, 38 V>D ,40 V>D, Ins 56 CCTATG, Ins 41 T, Ins97 A3, del42 G, del 43 A, del 41 C and del 42 A)