Expression of HCOV NL63 and 229E genes in Corona virus infections with elevation of serum Zinc levels

Abstract

A total of 100 serum samples were collected from patients with 20 nasal and throat swab samples of Corona virus and 100 samples as a control group from patients were recumbent in al-Yarmook and Al- Numan Hospitals from period 2st January 2021 to 1st September 2021. The mean of patient's ages were high compared to control in Corona virus infection (NS), the IgG of patients were high compared to healthy persons HS., the IgM mean was more than healthy persons HS. While the mean of serum zinc levels of patents a high compared to control HS. The mean of patient's ages with mild was HS, sever was (HS), and critical infection was (Sign), the IgG mild, sever was NS, and the critical also was NS. The IgM mean the mild was NS, the sever was HS, and the critical was HS. The mean of serum zinc levels with mild infection, sever and critical infection were NS, with the validity tests, serum zinc sensitivity 89%, Specificity94.1% positive predictive value (PPV) 93.4% and accuracy 91.6% P=0.00 (HS). Ct positive of genotyping was (24%) are low and the high positive 3(%) in patents have corona virus infections. The positive assay of NL63 gene (10.53%) isolated from nasal and throat swab patients have corona virus infections, the positive CT for NL63 gene (4%), also 229E gene (56.9%) isolated from nasal and throat swab. The NL63 and E229 genes expression was investigated in patients who were infected with Corona virus in comparison with the healthy controls by using real-time PCR. The results showed that there was high Ct value for patients and controls with high Ct value of templates, preoperational to the gene concentration.