TSP-1 level with (rs222826 T/C) Polymorphism in acute myeloid leukemia in Iraqi population

Abstract

Purpose: Acute myeloid leukemia patients from Iraq were studied to determine the prevalence and correlation of Thro mbospondin 1 (THBS1) gene single nuc leotide poly morphisms (SNPs). Method: Amplification Refractory Mutation System Polymerase Chain Reaction genotyping of the THBS1 gene SNPs (rs2228261 C>T) in a prospective case-control study of individuals with acute myeloid leukemia and healthy controls (ARMS PCR). Result : conducted a case– control study on 60patients and 30 healthy groups from the country of Iraq. Individuals was genotyped for SNPs ( rs2228261 C > T ) in THBS1 . The genotypic distributions of THBS1 in the case and control groups were examined for links to the development of cancer. Acute myeloid leukemia with odd ratio was show to be statistically significantly related with homozygous CC of THBS1 rs2228261 T > C, according to compared between the patients and control groups ( 0.068) ,while no found significant different in heterozygote pattern TC between patients with acute myeloid leukemia than control group with odd ratio ( 0.30).Also result found the level of TSP-1 show a significant decrease (p≤ 0.00) in the patients with AML as compared to the control. Conclusion: Iraq population genotyped for THBS1 gene SNPs using ARMS PCR only showed genetic link in homozygote pattern CC, which was more common in control groups than patients.