Assessment Adipokines gene expression and serum level of Chemerin in a sample of insulin resistance polycystic ovary Iraqi women

Abstract

The aim of this study is to detect the relation between Chemerin gene and polycystic ovary Iraqi women (PCOs), One hundred blood samples were taken, fifty samples were diagnosed as polycystic ovary cases, and the other fifty cases were healthy women, These samples were divided into two groups: the PCOs as patients group and the healthy people as a control group. Next the biochemical test were done for all samples such as blood glucose levels, insulin level lipid profile, Testosterone, E2, FSH , LH and Measurement of Chemerin level by ELISA. Then RNA extracted and converted to cDNA to detection gene expression for Chemerin gene by real time PCR. The data were then analyzed with Statistical Analysis System- SAS (2012) to detect the effect of difference factors in study parameters, Results. The women with PCOS showed significantly higher expression and level of Serum compared to healthy control women.