Association SNP (Rs10889677) In Mirnas Let-7f and Let-7e Binding Site of IL23R Gene with Colorectal Cancer

Abstract

The current study aims to Associate SNP (rs10889677) in miRNAs Let-7f and Let-7e binding site of IL23R gene with colorectal cancer in some Iraqi cases, The result of DNA- sequencing of the desired SNPs rs10889677 C >A, shown, the percentage of deletion mutation in patient was 33.33% while in control group 7.6% (p 0.014), deletion mutation was higher in male 20.8% than female12.5%, the percentage of deletion mutation was 29.16% in Adenocarcinoma, 4.16% in Mucinous adenocarcinoma, The distribution of deletion mutation in cancer grade, in the well differentiated the percentage of deletion mutation was 16.66% , in the midrate differentiated was 12.5% , in the Poor differentiated was 2.08%, The metastases of lymph node the deletion mutation, in the N0 was 16.66%, in the N1 8.33% , in the N2 was 2.08%, in the N2b was 6.25%, belong to cancer stage, the highest percentage in III (12.5%), in the I stage was (8.3%) , in the IIb (6.25%) and in the IIA stage was (4.16%) while no deletion mutation appeared in other stages include (II, IV, IIIC and IIC). According to metastasis; in the Mx stages the deletion mutation was (4.16%) whlile in the M1 the deletion mutation was (31.25%) , The result of genotyping shows three genotyping homozygotes (CC) and (AA), heterozygote (AC) the most common genotyping in patient AC with (18) in patient and (16) in control group, the second common genotyping in patient group was (CC) with frequency (9) and (2) in control group, the last genotyping was AA was more frequent in control group (6) than patient group (5), all of them were non- significant differences. The genotyping distribution shows no significant differences in all sub types, the AC was more frequent in Adenocarcinoma (9) in Mucinous adeno carcinoma AC was more frequent (6) , the AC was most common in the stages well differentiated was (11) while in the Moderate differentiated (6) and in the high grad (1) the Metastases of Lymph node showed AC was higher in N0 (9), in N2 was (4) and (2) in both N1 and N2a, the frequencies of CC in N0 and N1 was (3), in N2, N1b and N2b was (1) and absence in N2a. The AC was more frequency (16) in Metastases stages Mx while in M1 was (2), the current study conclusion that there was robust association of deletion mutation in this region but didn’t association of rs10889677 variation with colorectal cancer characterization.