Molecular Identification of Bacillus Sp. Isolated from Iraqi Soil and Efficiency of Antibiotics Production

Abstract

The present study aims to isolating and identification local bacterial isolates producing antimicrobials from some of the soil samples of Thi-Qar governorate and detecting the presence of 16SrRNA gene by using DNA sequence technique. This study included collected 25 soil samples from different areas of Thi-Qar governorate. A series of dilutions was done for each sample and was cultured on the Nutrient Agar Medium. The molecular study was carried out by extracting DNA from all isolated by using a simplified method requiring less effort and simpler and less expensive materials. The presence of 16SrRNA gene was detected by using special primers and all isolates were given a positive result of this gene  All isolates were tested for their ability to produce antimicrobial materials by taking tablets of isolates and streaking them on Muller Hinton Agar that cultured with one of pathogenic bacteria, which included Klebsiella pneumonia, Pseudomonas aeruginosa, Proteus mirabilis, Salmonella typhi and Staphylococcus aureus that identified by biochemical tests and VITEK 2 system.