Effects of Low Level Laser Irradiation on Blood Plasma and blood parameters of Breast Cancer patient

Abstract

This study designed to evaluate the effect of Low level laser in vitro on Some blood parameters such as Erythrocytes Sedimentation Rate, Mean cell volume, hematocrit and MCHC of patients’ with Breast Cancer by irradiated a human blood plasma samples by using a green laser and comparing this effects pre- and post- irradiated at fixed power density (100mW/cm2) and wavelength (532nm) of the laser that we used. The 8 ml of fresh blood samples collected in EDTA- tubes and separated in to four equal quantities: one for control (non-irradiated) sample and other three for irradiation by laser. The blood samples separated by bench centrifuge and cells sediment in the bottom of the tube and the plasma are supernatant in the up of the tube. After separated, the plasma irradiated by laser with different exposure time (10, 20, and 30) minutes the blood parameters are measured by using measured   Automatic hematology analyzer and then ESR values are measured by using a Westergren method. The RBCs volume (MCV) increased while MCHC are decreased and also the values of ESR decreased significantly at 30min exposure time.