The Association of Single Nucleotide Polymorphism in SLC30A8 Gene with Type 2 Diabetes Mellitus in AL-Najaf Population.

Abstract

Background: Diabetes mellitus is a collection of chronic endocrine disorders marked by persistent hyperglycemia caused by insulin production, insulin action, or both. The importance of insulin as an anabolic hormone leads to a metabolic deficit in carbs, lipids, and proteins. Various genome-wide association studies have identified several single nucleotide polymorphisms associated with type 2 Diabetes, as they were found to alter lipid metabolism, insulin secretion, glucose metabolism, and insulin receptor signaling, and the rs13266634 found in (the solute carrier family 30 member 8) SLC30A8 gene is one of the consistently reported risk factor single nucleotide polymorphism for type 2 Diabetes Study Objective: To verify the association of SLC30A8 gene single nucleotide polymorphisms and the risk of occurrence of type 2 Diabetes mellitus in AL-Najaf population and explore the role of SLC30A8 gene on insulin secretion. Methods: This case-control study enrolled 100 type 2 Diabetes mellitus patients and 100 controls who fulfilled the inclusion criteria. Blood samples were collected from all participants and were used for the rs13266634 single nucleotide polymorphism genotyping by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique. Outcomes: Two-hundred case-control studies with 100 cases and 100 controls were included for SLC30A8 and type 2 Diabetes mellitus. The comparison of results of parameters (fasting blood glucose, homeostatic model insulin resistance) exhibited significant (p<0.0001) increases in the patient’s group when compared with the group of controls. The comparison of results of insulin values in the patients group (P=0.103) insignificant decrease with respect to the controls group. Genotyping result of single nucleotide polymorphism (rs13266634 C/T) of Type 2 Diabetes mellitus as well as control persons under the co-dominant model showed that patients of heterozygous genotypes (CT) significantly elevated (OR = 2.56, 95% CI = 1.33 - 4.67, P = 0.0049) with respect to the control group. The dominant model indicated that patients of (CT+TT) genotypes increased significantly (OR=1.87, CI 95%=1.04 - 3.30, P=0.0434) with respect to the controls, and the single nucleotide polymorphism (rs13266634 C/T) of SLC30A8 not associated with of phenotypic parameter analysis. Conclusion: The rs13266634 is single nucleotide polymorphism significantly associated with type 2 Diabetes mellitus susceptibility among AL-Najaf Population and this gene not associated with insulin level, fasting blood glucose, and homeostatic model insulin resistance. Recommends: A large sample size is required to investigate the correlation between rs13266634 SNP of SLC30A8 gene and occurrence of disease and further studies in the future should be done on different SNPs of SLC30A8 gene in AL_Najaf population. These studies can discover which SNPs are more common in this governorate that involved in the pathogenesis of T2DM.