Pattern at the D16S539, PENTA D, PENTA E and TPOX loci by forensic analysis in Peru Southern region

Abstract

The study of genetic variability through microsatellite markers helps to characterize a population molecularly. Moreover, the genetic parameters obtained are useful in forensic statistical calculations, population genetics, and person identification. The present study aimed to identify the alleles and determine the allelic frequencies of the STR markers D16S539, PENTA D, PENTA E, TPOX present in the Southern Region of the Peru human population. Alleles were identified, allele frequencies of STRs were found, and it was determined if these markers were in Hardy-Weinberg genetic equilibrium. The study analyzed 100 samples from individuals who attended the Molecular Biology laboratory of the Universidad Nacional de San Agustín. DNA extraction was performed and amplified by PCR technique, the amplification products were visualized in polyacrylamide gels, and the computer analysis was done in the GENEPOP program. In the human population of the Southern Region of Peru, 4 alleles were identified for the Tpox marker, 5 alleles for the D16S539 STR, 8 alleles for the Penta D marker and 10 alleles were identified for the PENTA E marker; the STR with the highest discriminatory power was the Penta E STR. STRs markers TPOX, D16S539, PENTA E and Penta D are in Hardy Weinberg equilibrium in the human population of the Southern Region of Peru with a P<0.05 for all markers.