New Satellitism Test and molecular detection of haemophilus influenzae in Respiratory tract infections in mosul, Iraq

Abstract

Background: Haemophilus influenza fastidious microorganisms can not grow on blood agar but can be seen around beta-hemolyt ic colonies of S.aureus .In this study, we tested and compared with S.aureus and S.pyogenes to determine an alternative bacteria to be used for satellite test to identify H.influenzae .We collected 120 swabs from different respiratory tract infections the results show that 12(10%) of 102 positive culture was H.influenzae and 8(66.6%) were positive by satellite test with S.aureus and 11(91.6%) were positive by satellite test with Streptococcus pyogenes and 12(100%) approved by PCR assay as Haemophilus inflenzae. Based on our findings, the.pyogenes satellitism test is more reliable than the S.aureus test and produces fewer false-negative results.