Fetal Sex Determination Using Cell-Free Fetal DNA in Pregnant Women: Diwaniyha Local Study

Abstract

Objective :Our Interest has been sparked through the viable use of plasma and serum for molecular diagnostics. women's plasma and serum have each covered circulating DNA. and this substance has passed through molecular examination. We checked out whether or not fetal DNA become contribution with inside the maternal plasma and serum, that is a circumstance that takes place further all through pregnancy. Materials and methods: Plasma and serum were rapidly treated in order to extract the DNA from them. A tricky Y-PCR test was used to detect circulating male fetal DNA from pregnant girls carrying male babies using DNA from the plasma, serum, and nucleated blood cells of 43 pregnant girls. Results: In 40 maternal plasma samples, fetal-derived Y sequences had been found. Only 10 L of the samples had been used to get those findings. Only five (17%) of the 30 samples produced a wonderful Y sign while DNA from nucleated blood cells removed from a similar quantity of blood changed into employed. None of the 10 non-pregnant manipulate ladies, nor any of the thirteen ladies wearing woman babies, had wonderful plasma, serum, or nucleated blood tests. Conclusion: We concluded that free circulating fetus DNA could be used for gender determination or other possible molecular studies related to the fetus during pregnancy period.