Identification and Evaluating of Phytochemicals Extracted from Mirabilis Jalapa (L) Leafs on Some Antibiotic Resistance Bacteria

Abstract

This study was conducted in the laboratories of the faculty of Science / University of Kufa from October 2021 to March 2022, the Mirabilis jalapa(L.) plant was obtained from some gardens in Nagif City , A specific weight (10) grams of leaf powder was extracted separately by Maceration and vortex method using methanol 96% with three temperatures (30,40 and 50°C) degrees to obtain the best alcoholic extract . The qualitative analysis was studied for the detection phytochemicals using chemical reagents and GC-MS analysis was conducted to identify the quality and quantity of phytochemical compounds in crude extraction methods used in this study. The extracts were chosen from the followed methods depending on the amount of active compounds in each extract diagnosed by GC-MS. Where the results of chemical tests for the alcoholic extract showed positive results for alkaloids using, phenols, terpenes, alkaloids and glycosides .Result show the alcoholic extract of the leaves of the plant using the Maceration method and a temperature of 30 °C showed the highest rate of the chemical compounds diagnosed using GC-MS and were chosen as the best treatments in the subsequent experiments. Bacteria resistant to antibiotics study were obtained from the post Graduate Microbiology Laboratory / College of Science / University of Kufa. 15 bacterial samples were diagnosed by morphological, microscopic and biochemical tests. The diagnosis was confirmed using the Phytek device, and the percentage of gram positive was as follows S.aureus (48.57%), followed by P.aeruginosa (37.14%). Three concentrations of leafs extracts by Maceration 30°C as 1, 5 and 10 mg/ml were prepared for each of the selected extraction methods to evaluate the antibacterial activity at three replicates for each concentration using the geavure diffusion method.