Fabrication and Evaluation of Antibacterial Analysis of a Seaweed Biopolymer Based Hydrogel for Periodontal Therapeutic Applications

Abstract

AIM : The aim of the study is to estimate the biological and mechanical properties of hydrogel fabricated from the mixture of sea weeds. Introduction: Hydrogel is a promising material for local antimicrobial application. Hydrogel refers to a kind of biomaterial synthesized by either synthetic or biopolymer. Carrageenan is used for coughs, bronchitis, tuberculosis, and intestinal problems. The ability to combine agar with chitosan and carrageenan with inorganic substances such as tricalcium phosphates and hydroxyapatite makes these matrices also useful for oral delivery, burn wound healing and bone TE. Chitosan has potent, much adhesion, homeostatic action, film-forming ability, biodegradability and anti-microbial action.  Materials and methods: In a flask 0.1g of chitosan is added to the distilled water and heated for an hour. Meanwhile the solubility is checked. After checking the solubility of 0.1g of Agar, Rutin and 0.2g of carrageenan added to 100 ml of chitosan. Mixed solution is kept Ina magnetisms stirrer for 1 hour at 100 degree Celsius. Anti-microbial activity : Agar well diffusion and Disc diffusion method is used. Then incubated for 37 degrees Celsius for 48 hours. The zone of inhibition is recorded. Mechanical properties: (FTIR) Fourier transform infrared and contact angle were measured Results: The results showed that the hydrogel formed from the mixture of seaweed has potential antimicrobial activity against streptococcus mutans as the concentration increases and it also showed that the physical properties of the hydrogel derived from seaweed had more promising characteristics as the concentration increases. Conclusion: Seaweed will provide striking bioactives, which can be used as a promising medicine for the treatment of human diseases, orelse it can be used as new antimicrobial agents to be the replacement for synthetic antibacterial agents used in various applications. Hydrogel prepared from the mixture of seaweeds can be used in the application of drug delivery; it had shown that it had promising antimicrobial activity and physical properties.