Estimation of Orexin Level and Some Biochemical Indicators in Pregnant Women with Normal Pregnancy and Pregnant Women After Taking Steroids

Abstract

The study measures the level of orexin and a number of biochemical variables, including cholesterol, triglycerides, high-density lipoproteins, low-density lipoproteins, very low density lipoproteins. In addition, it explores the total antioxidant index. It also measures the level of Malondialdehyde in pregnant women with normal pregnancy, as well as pregnant women after taking steroids and non-pregnant women after taking steroids. The samples were divided into 40 pregnant women with natural pregnancy as a control group, (30) samples for pregnant women after taking steroids, and (30) samples for non-pregnant women after taking steroids and in different age groups (15-45) years. Blood samples were collected from women visiting Azadi Teaching Hospital as well as outpatient medical gynecological clinics for the period from 12/25/2021 to 15/5/2022. The study showed:* There was an increase with no statistical differences at the probability level (p> 0.05) in the level of orexin for the age group (26-35) years in pregnant women with normal pregnancy and pregnant women after taking steroids compared with the two age groups (15-25), (36-45). Yet, it increased with no statistical differences in the age group (36-45) years in non-pregnant women after taking steroids compared with the two age groups (15-25) years (26-35) year. * There was an increase no statistical differences at the level of probability (P> 0.05) in the MDA for the three age groups respectively for pregnant women with steroids and non-pregnant women after taking steroids compared with pregnant women with natural pregnancy. The highest percentage was in the age group (36-45) years for both pregnant women with natural pregnancy and pregnant with steroids and non-pregnant women after taking steroids compared with the age group (15-25) and (26-35) years. * There was a decrease with high statistical differences at the probability level (P≤0.01) in the level of TAC in the age group (36-45) years for pregnant women with a normal pregnancy more than the two age groups (15-25) and (26-35) years. The same happened with the two groups (pregnant women after taking steroids and non-pregnant women after taking steroids). * There were no statistically significant differences at the probability level (p>0.05) in the TC of the three age groups in blood serum of pregnant and non-pregnant women and pregnant women with steroids. * There was an increase with high statistical differences in the level of TG, VLDL at the probability level (p ≤ 0.01) for the three age groups in the blood sera of pregnant women with normal pregnancy and steroids compared with the age groups of the non-pregnant group. * There was a decrease but no statistical differences appeared at the probability level (p > 0.05) in the level of HDL in the serums of pregnant women with normal pregnancy and pregnant women after taking steroids and non-pregnant women after taking steroids for the three age groups. Here, the highest percentage decrease was recorded in the age group (G3 = 36-45).* There was an increase with no statistical differences at the probability level (p> 0.05) in the level of (LDL) in the serums of pregnant women with normal pregnancy and pregnant women after taking steroids compared with non-pregnant women after taking steroids for the three age groups. The highest percentage of increase was recorded in the age group (G3 = 36-45).