Association Between HER2Ile655Val Single Nucleotide Polymorphism and the Risk of Breast Cancer in the Kurdish Population

Abstract

Introduction: The human epidermal growth factor receptor-2 (Her2) is a transmembrane protein responsible to regulate the growth and proliferation of breast cell with tyrosine kinase activity. Researchers have looked at the possibility that HER2Ile655Val has a role in generating of breast cancer. The genetic variants of HER2 and single nucleotide polymorphism have been explored as being connected with breast tissue neoplasia; however, research of this sort has not been carried out on the Kurdish community. The present study was aimed to investigate the role of HER2 gene polymorphism in the risk of breast cancer development in Kurdish population in Iraq and it is the first of its kind in the study area. Methods: We analyzed the distribution and frequency of Val/Val and Ile/Val genotype from blood of 151 patients who have cancer in breast tissue and 121 healthy individual controls using the combination of PCR and Restriction fragment length polymorphism, PCR-RFLP. Results: The result of this study found that there is twofold increase in the risk of malignant breast tumor in Kurdish women who carries HER2^Ile655Val genotypes, Val/Val and Ile/Val (P<0.005). The rate of HER2^Ile655Val polymorphism was 63.3% among breast cancer patients and this is the highest rate globally after Greek. Older ages, >45, recorded the highest rate of polymorphism (P<0.005) among patients, 62.5%. Conclusion: This result concluded that there is an association between HER2 codon 655 polymorphisms with the risk of breast cancer among Kurdish population.