Shareef Saleh Al-khateeb Nawfal Hussein Aldujaili


The purpose of this research was to study the biological activity of Magnesium oxide against XDR bacteria Pseudomonas aeruginosa and Staphylococcus aureus MARSA that isolated from burns infections. During the months of August 2022 to December 2022, a total of 75 specimens and investigations were collected. Specimens for burns infection were gathered from two hospitals in the province of AL-Najaf (AL-Hakeem Hospital and Central Laboratory in najaf ). Swabs from burns infection patients were collected and sent to the lab, where they were streaked on MacConkey agar, a blood agar., Gram-negative bacteria had a high rate of 58 (78%) based on morphological and biochemical tests, with P. aeruginosa having a high percentage of 39 (52 %) and S. aureus having a high percentage of 15 (20 %). K. pneumonia has 9 (12 %), E. coli has 6 (8%), B.cepacia 3 (4%) , A.baumannii 2(2.67%) and other in 1 (1.33%) . while G +ve bacteria recorded 17 (22 %), with S. aureus being the most isolated bacterium in this investigation. The first diagnosis of bacterial isolates was based on characteristics such as Gram stain, morphology, biochemical testing and automated VTEK-2 . Isolated bacteria were tested against E. coli as an indicator strain for Magnesium Oxide Nanoparticle production. The most effective isolate was chosen based on the colors that change and formation of white precipitation and the biological activity of Mgo NPS against XDR bacteria. B. subtitles S3, was the most effective isolate. Because each microbe has a unique metabolic process and enzyme activity, not all bacteria can manufacture NPs . The characterization of biogenic Mgo NPs was achieved, using UV visible spectrophotometry, Field Emission Scanning electron microscope (FESEM), Energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), and Atomic force microscope (AFM).

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Nanotechnology , Antibacterial activity of Mgo , XDR bacteria , Anti-biofilm activity of Mgo .

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