Role of Tumor Necrosis Factor – Α ( TNF – Α) (G308A) Gene Polymorphism and Levels in Coronary Artery Patients in thi-Qar Province

Abstract

The present study was carried out in the labs of Technical Institute –Al-Nasiriyah /Community Health Department and Nasiriyah Heart Center in Thi-Qar province, during the period of research was extended from January to July 2022. The aim of study was to determination polymorphisms of TNF-α gene in patients with Coronary Artery Disease and measuring its levels in serum using a technique enzyme-linked immune sorbent adsorptive (ELISA). The study included a total of 100 Iraqi patients with coronary artery disease (CAD) and healthy control group their age between 3-85 years. DNA has been isolated and PCR was performed by using primers specific for genotypes of TNF-α gene(308 A/G). The results showed that Gel Electrophoresis of PCR amplified TNFα-308 product with molecular size 107 bp. Frequency of genotypes for the TNF-α gene showed a high frequency of GG genotype compared to the AG and AA genotypes in all study groups, with no significant differences between cardiovascular patients and control group. The immunological study showed that there was a significant increase of concentrations of (TNF-α) in patients compared to the healthy control group. Thus it seems There is no correlation between TNF-α gene polymorphisms and the risk of coronary artery disease in Thi- Qar province and the Serological Assay (ELISA) showed a significant increase in the concentration of TNF-α in patients with coronary artery disease compared with the healthy control group.