Alcoholic Rosmarins Officinalis: Antibacterial Activity Aganist Local Isolates Staphylococcus Aureus

Abstract

The current research included isolation and identification of the active compounds of rosemary plant. Soxhlet was used in the separation process using an alcoholic solvent (ethanol at a concentration of 70%). The detection and identification of the active compounds were accomplish out using gas chromatography - mass spectrometry.(Bicyclo[3.1.1]hept-3-en-2-one, 4,6,6-trimethyl-, (1S)-and 11-Octadecenoic acid, methyl ester and cis-13-Octadecenoic acid), and the antibacterial of the alcoholic extract against some five Pathogenic bacterial isolates, as Staphylococcus aureus that isolated from many infection by the test was accomplishby using wells diffusion method as well as measurement the optical density of bacteril growth of spectrophotometer, the results showed that increasing of average inhibition zone for(50 ,100 ,150 , 200)mg\ml alcoholic plant extract was non-significant between all concentration . while the bacterial growth for S.aureus be significant effected during Logartimic phase compare with control . The conclusion of the research was alcoholic Rosemary extract have impact inhibition growth Staphylococcal isolate , also we can recommend this plant extract replacement of common antibiotics.