Polymorphism AR-E211 G>A of the AR Gene (G1733A) In Patients with Prostate Cancer (Pca) In Middle Euphrates of Iraq

Abstract

The pathogenesis of prostate cancer (CaP) involves alterations in a gene structure of the androgen receptor (AR). The single nucleotide polymorphism AR-E211 G>A localized in exon 1 of the AR gene (G1733A) was detected using direct polymerase chain reaction and restriction digestion (PCR-RFLP) method on blood samples with prior DNA isolation. We used blood samples of patients with a diagnosis of CaP. From monitored group of CaP patients were selected specimen in formalin-fixed paraffin-embedded tissue blocks with morphology of CaP. The main objective of our study was to develop a method based the direct PCR-RFLP analysis from blood with prior DNA isolation for faster genotyping analysis of a large number of samples. We found no statistically significant differences in allelic % of the AR-E211 G>A polymorphism between healty and CaP patients. Genotyping of the AR-E211 G>A variant in blood was not identical with tumor tissue genotyping analysis. Although we analyzed a limited number of the patient samples, we suppose that a presence of the minor allele A may be associated with cancer transformation-induced changes of the modified AR gene.