Estimation of the Genetic Dimension of Candida Isolates Isolated from Pathological Cases by RAPD PCR Method

Abstract

background Yeast is a type of fungus. People who take antibiotics may develop candidiasis because the antibiotics kill off naturally occurring bacteria in the body, which helps Candida grow unchecked, Treatment with corticosteroids or drugs that suppress immunity can also reduce the body's defenses against Candida. Methodology100 samples were isolated from people suffering from urinary tract infections using sterile swaps and isolated on their selective media All clinical samples of suspected lesions were infected with chloramphenicol on SDA Cycloheximide supplementation intermediate using a sterile inoculation ring and incubated at 25-30°C before being assessed. Negative cultures were after 4 weeks, whereas positive cultures were inspected by macroscopic and microscopic identification. Result The efficiency ratios of the primers OP-V19, OP-L05, OP-M06, OP-M14, OP-E20, OP- L20, OP-M20, OP-P04, OP-V14 and OP-l02 were 5.797, 11.594, 8.695, 10.144, 8.69, 5.79, 10.14, 11.59, 1.897019 and 3. 17.39 so that the best primer was OP-l02 because the number of bundles recorded for this bundles was 12 recorded bundles whose sizes ranged between 200 -3000 base pairs featured by the presence of six public bundles 200, 700, 900, 1200, 2000 and 3000 and present six differential bundles 500, 550, 600, 650,1500 and 2200 base pairs. It comes in second place OP-P04 because the number of bundles recorded for this bundle was 8 recorded bundles whose sizes ranged between 225 -1600 base pairs Featured by the presence of two public bundles 350 and 500, and present 6 differential bundles 225, 550, 600, 85, 1200 and 16000 base pairs.