Real Time-PCR for the detection of the Leishmania sp. and genomic sequencing for the detected the parasite in Babylon, Iraq

Abstract

The most frequent type of leishmaniasis to infect people is cutaneous leishmaniasis (CL). A single-celled parasite that is spread by the bite of a Phlebotomine sp. sand fly causes a skin lesion. There are around thirty different kind of Leishmania sp., all of them can cause cutaneous leishmaniasis. In many parts of Iraq, it is one of the most prevalent parasitic infections. This significantly hinders the socioeconomic development of this impoverished province. The samples current study was skin snip and skin swab which are diagnosed through RT-PCR for the detection of the parasite and gene sequencing done for the detected parasite, The result shows that 45 (55.6) was positive microscopical and 36 (44.4) was negative while 53 (65.4%) Positive result of Nested multiplex PCR. The objective of the current study was to molecularly identify the different species of cutaneous leishmaniasis in Babylon, Iraq, patients.